The precipitated protein can be re-dissolved readily in detergent containing buffers. Low protein concentration samples can be effectively precipitated and concentrated in about 25-30 minutes. It is simple, rapid and highly effective. In order to overcome these shortcomings, we have developed this High Efficiency Protein Precipitation Kit, which is a modification of traditional TCA method. The higher protein concentration sample can be precipitated much more effectively than the lower protein concentration samples. Another major disadvantage of traditional TCA/acetone precipitation method is its low efficiency for the samples of low protein concentration. However, proteins precipitated by TCA method is usually denatured and in many cases the solubility of protein is reduced. One of the most commonly used methods is trichloroacetic acid (TCA) precipitation. Protein precipitation is an option for concentrating proteins and removing interfering substances found in protein samples such as salts, lipid and other components that may interfere with downstream applications. c, NISTmAb in 12.5 mM L-histidine, 12.5 mM L-histidine HCl (pH 6.0). Protein extracts were precipitated with 6 volumes of cold acetone and kept at -20C overnight, followed by two pellet-washing steps, each with cold acetone. Acetone precipitation One-step acetone precipitation was performed as described recently 17. This kit can simply precipitate protein in 25 minutes with high efficiency. In the buffer containing harsh detergents, protein signal is attained only with precipitation. for a short duration (13 min) and dissolved in a buffer of choice for protein analysis.
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